KMID : 0364820120480040284
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Korean Journal of Microbiology 2012 Volume.48 No. 4 p.284 ~ p.292
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Characteristics and Virulence Assay of Entomopathogenic Fungus Nomuraea rileyi for the Microbial Control of Spodoptera exigua (Lepidoptera: Noctuidae)
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Lee Won-Woo
Shin Tae-Young Ko Seung-Hyun Choi Jae-Bang Bae Sung-Min Woo Soo-Dong
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Abstract
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To date, chemical control remains the most common way to reduce beet armyworm (Spodoptera exigua)
populations. However, this insect has become more tolerant or resistant to many chemical insecticides and the insect
larvae usually hide inside hollow, tube-like leaves of host plant so they were difficult to kill by spraying insecticides.
The use of viral and bacterial insecticide to solve these problems has not been successful because of their novel
feeding habit. To overcome these problems, in this study, the biological characteristics and virulence of an
entomopathogenic fungus isolated from the cadaver of larvae beet armyworm were investigated. Isolated
entomopathogenic fungus was identified as Nomeraea rileyi (Farlow) Samson by morphological examinations and
genetic identification using sequences of the ITS, ¥â-tubulin gene and EF1-¥á regions. This fungus was named as N.
rileyi SDSe. Virulence tests against 3rd larvae of beet armyworm were conducted with various conidial suspensions
from 1¡¿104 to 108 conidia/ml of N. rileyi SDSe in laboratory conditions. Mortality rate of beet armyworm showed
from 20 to 54% and the virulence increased with increasing conidial concentrations. Although N. rileyi SDSe
showed low mortality rate against beet armyworm, it is expected that N. rileyi SDSe will be used effectively in the
integrated pest management programs against the beet armyworm.
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KEYWORD
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Nomuraea rileyi, Spodoptera exigua, biocontrol, entomopathogenic fungi
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